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AC16 Human Cardiomyocyte Cell Line: SYBR GoldTM-labeled (Thermo Fisher Scientific, Inc.), and TMRM-labeled images
- Citation Author(s):
-
Chan-MinHsu
National Taiwan University - Submitted by:
- Chan-Min Hsu
- Last updated:
- Sat, 11/20/2021 - 02:18
- DOI:
- 10.21227/ckc7-2t42
- Data Format:
- License:
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- Keywords:
Abstract
This dataset includes 70 sets of 3D confocal high-resolution images. All images were imaged using an LSM800 Zeiss microscope with a Plan-apochromat 1.40-NA, 63× objective, and Zeiss ZEN Blue 2.6 software was used to acquire the images. Three channels were used to acquire transmitted light (TL), SYBR GoldTM- (Thermo Fisher Scientific, Inc.) labeled (nuclear and mitochondrial DNA), and TMRM-labeled (mitochondria) images. Each confocal image consists of 32 slices with an interval of 0.15 µm and a YX resolution of 917 × 917 pixels.
The datasets are transformed into mask images by conducting data preprocessing, including histogram equalization, background subtraction, Gaussian noise removal, Sigma filtering, gamma adjustment, and CLAHE.
46 samples were used as a training set (/train/Confocal), 20 samples were used as a testing set (/test/Confocal), and 4 samples were used as a validation set (/val/Confocal).
The confocal images were transformed into mask images and separated into /source, /target, /target_dna in each directory (/train, /test, /val).
Comments
I would like to use this dataset for my research work.